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Detection of protein alterations in male breast cancer using two dimensional gel electrophoresis and mass spectrometry: the involvement of several pathways in tumorigenesis.

Identifieur interne : 000047 ( Main/Exploration ); précédent : 000046; suivant : 000048

Detection of protein alterations in male breast cancer using two dimensional gel electrophoresis and mass spectrometry: the involvement of several pathways in tumorigenesis.

Auteurs : Karim Chahed [Tunisie] ; Maria Kabbage ; Bechr Hamrita ; Christelle Lemaitre Guillier ; Mounir Trimeche ; Sami Remadi ; Laurence Ehret-Sabatier ; Lotfi Chouchane

Source :

Clinica chimica acta; international journal of clinical chemistry [ 0009-8981 ] ; 2008.

RBID : pubmed:17996735

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence (MeSH), Breast Neoplasms, Male (metabolism), Breast Neoplasms, Male (pathology), Cell Transformation, Neoplastic (metabolism), Cell Transformation, Neoplastic (pathology), Electrophoresis, Gel, Two-Dimensional (MeSH), Humans (MeSH), Male (MeSH), Middle Aged (MeSH), Molecular Sequence Data (MeSH), Neoplasm Proteins (chemistry), Neoplasm Proteins (metabolism), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH).
MESH :
- chemical , chemistry : Neoplasm Proteins.
- metabolism : Breast Neoplasms, Male, Cell Transformation, Neoplastic, Neoplasm Proteins.
- pathology : Breast Neoplasms, Male, Cell Transformation, Neoplastic.
- Amino Acid Sequence, Electrophoresis, Gel, Two-Dimensional, Humans, Male, Middle Aged, Molecular Sequence Data, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.

Abstract

BACKGROUND

Little emphasis has been placed today on the elucidation of protein alterations in male breast carcinogenesis.

METHODS

Protein extracts were subjected to both isoelectric focusing (IEF) and non-equilibrium pH gradient electrophoretic (NEPHGE) analyses. Differentially expressed proteins in tumor tissues were identified by matrix assisted laser desorption /ionization time of flight (MALDI-TOF) mass spectrometry and database search.

RESULTS

Some of the alterations involve variations in the expression of cytokeratins 8, 18 and 19. More interestingly, tropomyosin1, a protein known to play a role in suppression of the malignant phenotype, was found to be under-expressed in cancer tissues, implicating a possible pivotal role for this protein in male breast carcinogenesis. Co-upregulation of molecular chaperones (heat shock protein HSP27 and protein disulfide isomerase), stress related proteins (peroxiredoxin 1 and peptidylprolyl isomerase A) and glycolytic enzymes (enolase 1) occurred also in male breast tumors. Some of the remaining alterations include proteins involved in invasion and metastasis, such as galectin 1 and cathepsin D.

CONCLUSIONS

The present study represents a first proteomic investigation of protein alterations in infiltrating ductal carcinomas (IDCA) of the male breast. A number of protein alterations in tumor tissues have been characterised thus, providing new insights into the molecular mechanisms underlying this disease.

DOI: 10.1016/j.cca.2007.10.018
PubMed: 17996735

Affiliations:

Tunisie

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Le document en format XML

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<affiliation wicri:level="1"><nlm:affiliation>Laboratoire d'Immuno-Oncologie Moléculaire, Faculté de Médecine de Monastir, Monastir, Tunisia. k.chahed@yahoo.fr</nlm:affiliation>
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<author><name sortKey="Kabbage, Maria" sort="Kabbage, Maria" uniqKey="Kabbage M" first="Maria" last="Kabbage">Maria Kabbage</name>
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<author><name sortKey="Ehret Sabatier, Laurence" sort="Ehret Sabatier, Laurence" uniqKey="Ehret Sabatier L" first="Laurence" last="Ehret-Sabatier">Laurence Ehret-Sabatier</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Detection of protein alterations in male breast cancer using two dimensional gel electrophoresis and mass spectrometry: the involvement of several pathways in tumorigenesis.</title>
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<affiliation wicri:level="1"><nlm:affiliation>Laboratoire d'Immuno-Oncologie Moléculaire, Faculté de Médecine de Monastir, Monastir, Tunisia. k.chahed@yahoo.fr</nlm:affiliation>
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<author><name sortKey="Trimeche, Mounir" sort="Trimeche, Mounir" uniqKey="Trimeche M" first="Mounir" last="Trimeche">Mounir Trimeche</name>
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<author><name sortKey="Ehret Sabatier, Laurence" sort="Ehret Sabatier, Laurence" uniqKey="Ehret Sabatier L" first="Laurence" last="Ehret-Sabatier">Laurence Ehret-Sabatier</name>
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<author><name sortKey="Chouchane, Lotfi" sort="Chouchane, Lotfi" uniqKey="Chouchane L" first="Lotfi" last="Chouchane">Lotfi Chouchane</name>
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<series><title level="j">Clinica chimica acta; international journal of clinical chemistry</title>
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<term>Breast Neoplasms, Male (metabolism)</term>
<term>Breast Neoplasms, Male (pathology)</term>
<term>Cell Transformation, Neoplastic (metabolism)</term>
<term>Cell Transformation, Neoplastic (pathology)</term>
<term>Electrophoresis, Gel, Two-Dimensional (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Male (MeSH)</term>
<term>Middle Aged (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Neoplasm Proteins (chemistry)</term>
<term>Neoplasm Proteins (metabolism)</term>
<term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Adulte d'âge moyen (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Humains (MeSH)</term>
<term>Mâle (MeSH)</term>
<term>Protéines tumorales (composition chimique)</term>
<term>Protéines tumorales (métabolisme)</term>
<term>Spectrométrie de masse MALDI (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Transformation cellulaire néoplasique (anatomopathologie)</term>
<term>Transformation cellulaire néoplasique (métabolisme)</term>
<term>Tumeur du sein de l'homme (anatomopathologie)</term>
<term>Tumeur du sein de l'homme (métabolisme)</term>
<term>Électrophorèse bidimensionnelle sur gel (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Neoplasm Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Transformation cellulaire néoplasique</term>
<term>Tumeur du sein de l'homme</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Protéines tumorales</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Breast Neoplasms, Male</term>
<term>Cell Transformation, Neoplastic</term>
<term>Neoplasm Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines tumorales</term>
<term>Transformation cellulaire néoplasique</term>
<term>Tumeur du sein de l'homme</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Breast Neoplasms, Male</term>
<term>Cell Transformation, Neoplastic</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Electrophoresis, Gel, Two-Dimensional</term>
<term>Humans</term>
<term>Male</term>
<term>Middle Aged</term>
<term>Molecular Sequence Data</term>
<term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Adulte d'âge moyen</term>
<term>Données de séquences moléculaires</term>
<term>Humains</term>
<term>Mâle</term>
<term>Spectrométrie de masse MALDI</term>
<term>Séquence d'acides aminés</term>
<term>Électrophorèse bidimensionnelle sur gel</term>
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<front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b>
</p>
<p>Little emphasis has been placed today on the elucidation of protein alterations in male breast carcinogenesis.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>METHODS</b>
</p>
<p>Protein extracts were subjected to both isoelectric focusing (IEF) and non-equilibrium pH gradient electrophoretic (NEPHGE) analyses. Differentially expressed proteins in tumor tissues were identified by matrix assisted laser desorption /ionization time of flight (MALDI-TOF) mass spectrometry and database search.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>Some of the alterations involve variations in the expression of cytokeratins 8, 18 and 19. More interestingly, tropomyosin1, a protein known to play a role in suppression of the malignant phenotype, was found to be under-expressed in cancer tissues, implicating a possible pivotal role for this protein in male breast carcinogenesis. Co-upregulation of molecular chaperones (heat shock protein HSP27 and protein disulfide isomerase), stress related proteins (peroxiredoxin 1 and peptidylprolyl isomerase A) and glycolytic enzymes (enolase 1) occurred also in male breast tumors. Some of the remaining alterations include proteins involved in invasion and metastasis, such as galectin 1 and cathepsin D.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b>
</p>
<p>The present study represents a first proteomic investigation of protein alterations in infiltrating ductal carcinomas (IDCA) of the male breast. A number of protein alterations in tumor tissues have been characterised thus, providing new insights into the molecular mechanisms underlying this disease.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>Tunisie</li>
</country>
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<tree><noCountry><name sortKey="Chouchane, Lotfi" sort="Chouchane, Lotfi" uniqKey="Chouchane L" first="Lotfi" last="Chouchane">Lotfi Chouchane</name>
<name sortKey="Ehret Sabatier, Laurence" sort="Ehret Sabatier, Laurence" uniqKey="Ehret Sabatier L" first="Laurence" last="Ehret-Sabatier">Laurence Ehret-Sabatier</name>
<name sortKey="Guillier, Christelle Lemaitre" sort="Guillier, Christelle Lemaitre" uniqKey="Guillier C" first="Christelle Lemaitre" last="Guillier">Christelle Lemaitre Guillier</name>
<name sortKey="Hamrita, Bechr" sort="Hamrita, Bechr" uniqKey="Hamrita B" first="Bechr" last="Hamrita">Bechr Hamrita</name>
<name sortKey="Kabbage, Maria" sort="Kabbage, Maria" uniqKey="Kabbage M" first="Maria" last="Kabbage">Maria Kabbage</name>
<name sortKey="Remadi, Sami" sort="Remadi, Sami" uniqKey="Remadi S" first="Sami" last="Remadi">Sami Remadi</name>
<name sortKey="Trimeche, Mounir" sort="Trimeche, Mounir" uniqKey="Trimeche M" first="Mounir" last="Trimeche">Mounir Trimeche</name>
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<country name="Tunisie"><noRegion><name sortKey="Chahed, Karim" sort="Chahed, Karim" uniqKey="Chahed K" first="Karim" last="Chahed">Karim Chahed</name>
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Detection of protein alterations in male breast cancer using two dimensional gel electrophoresis and mass spectrometry: the involvement of several pathways in tumorigenesis.

Detection of protein alterations in male breast cancer using two dimensional gel electrophoresis and mass spectrometry: the involvement of several pathways in tumorigenesis.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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